Journal: Neural Regeneration Research
Article Title: Matrine promotes neural circuit remodeling to regulate motor function in a mouse model of chronic spinal cord injury
doi: 10.4103/1673-5374.259625
Figure Lengend Snippet: Matrine increases neurofilament-H (NF-H)-positive axons in injured spinal cord. Sagittal sections of the spinal cords of vehicle-treated or matrine-treated were immunostained for NF-H. (A) Representative images at rostral and caudal sits are shown. Scale bar: 25 μm. (B) The diagram of analyzed area is shown. The measured areas in rostral and caudal sites were defined as the area 2.0–3.0 mm apart from the lesion center. (C, D) Ratio of NF-H-positive area to measured area was quantified at the rostral (C) and the caudal site (D). * P < 0.05, unpaired t -test (two-tailed). n = 5 mice (vehicle) or 6 mice (matrine).
Article Snippet: The slices were post-fixed with 4% PFA for 60 minutes at room temperature and immunostained with a chicken anti-neurofilament-H (NF-H, a marker for axons) polyclonal antibody (1:1000; Merck, Kenilworth, NJ, USA; Cat#AB5539), a rabbit anti-glial fibrillary acidic protein (GFAP, a marker for reactive astrocytes) polyclonal antibody (1:1000; Merck; Cat#AB5804), a goat anti-choline acetyltransferase (ChAT, a marker for motor neurons) polyclonal antibody (1:200; Merck; Cat#AB144P), and/or a mouse anti-bassoon (a marker for presynapses) monoclonal antibody (clone# SAP7F407; 1:2000; abcam, Cambridge, UK; Cat#ab82958).
Techniques: Two Tailed Test